Tenascin-N (TNN3) is a crucial extracellular matrix glycoprotein that plays an essential role in cellular adhesion, migration, and differentiation. The availability of a Human TNN3 cDNA Clone (10 µg) is significant for research in tissue development, cancer metastasis, and neurological function. This article explores the biological importance of TNN3, its applications, and how researchers can utilize this cDNA clone in their studies.
What is TNN3?
TNN3 is a member of the tenascin family, which is involved in modulating cell interactions within the extracellular matrix. It is primarily expressed in developing tissues and plays a role in neural patterning and tissue remodeling. For more information on tenascin function, visit NCBI and NIH’s National Library of Medicine.
Applications of Human TNN3 cDNA Clone
1. Cancer Research
TNN3 has been linked to tumor microenvironments and metastasis in various cancers. Studies have shown that it can contribute to cellular invasion and resistance to therapy. The National Cancer Institute and NCBI Cancer Research Database provide comprehensive data on tenascin-related oncogenesis.
2. Neurological Function and Disorders
TNN3 is expressed in the nervous system and contributes to axonal growth and synaptic plasticity. It has been implicated in neurodevelopmental disorders and neurodegeneration. Research at NIH Neuroscience Division and PubMed Central details ongoing studies on tenascin’s role in brain development.
3. Tissue Engineering and Regenerative Medicine
As a key extracellular matrix protein, TNN3 is used in biomaterials for regenerative medicine. It promotes cell adhesion and matrix remodeling in tissue grafts. Institutions such as the NIH Stem Cell Research and FDA’s Center for Biologics have reported its relevance in regenerative therapies.
4. Wound Healing and Fibrosis Studies
TNN3 has been shown to regulate fibroblast activity in wound healing and fibrosis, making it an essential component in studies on tissue repair. The USDA’s National Animal Health Laboratory Network and NIH Dermatology Branch provide data on extracellular matrix dynamics in healing.
5. Cardiovascular Research
Recent studies indicate that TNN3 is involved in heart tissue remodeling and may play a role in cardiovascular diseases such as fibrosis and hypertrophy. Research at NIH Cardiovascular Division has further investigated its potential as a therapeutic target.
6. Musculoskeletal Development and Disorders
TNN3 is essential in musculoskeletal formation and repair, impacting conditions such as osteoarthritis and muscle regeneration. Research at the National Institute of Arthritis and Musculoskeletal and Skin Diseases explores tenascin’s role in joint health and orthopedic applications.
7. Role in Developmental Biology
TNN3 expression is tightly regulated during embryogenesis, influencing organ development and cell migration patterns. The National Institute of Child Health and Human Development offers insights into tenascin’s developmental functions.
Production and Quality Control
Researchers using the Human TNN3 cDNA Clone must ensure high purity and sequence accuracy. The National Institute of Standards and Technology (NIST) provides guidelines for quality control of genetic materials. Additionally, laboratories must comply with biosafety regulations from the Centers for Disease Control and Prevention (CDC).
Comparative Analysis: TNN3 in Humans vs. Other Species
Comparative genomic studies indicate that TNN3 expression varies across species, affecting its function in different biological contexts. Resources from NCBI’s Comparative Genomics Division and NIH Comparative Biomedical Sciences offer insights into these evolutionary differences.
Potential for Personalized Medicine
With advances in genomic sequencing, TNN3 is being explored as a biomarker for disease susceptibility and response to therapy. The National Human Genome Research Institute and NIH Precision Medicine Initiative provide key insights into using genetic data for tailored medical treatments.
How to Obtain the Human TNN3 cDNA Clone
Scientists can acquire high-quality TNN3 cDNA clones from repositories such as ATCC (American Type Culture Collection) and BEI Resources, supported by the National Institute of Allergy and Infectious Diseases (NIAID).
Conclusion
The Human TNN3 cDNA Clone (10 µg) is an essential tool for biomedical and translational research. It enables investigations into cancer biology, neurodevelopment, regenerative medicine, cardiovascular disease, musculoskeletal disorders, and embryonic development. By leveraging resources from .edu and .gov domains, researchers can advance their studies and develop novel therapeutic strategies.
For additional information, visit the NIH Genetic Database, PubMed Central, the World Health Organization’s Research Division, and the National Center for Biotechnology Information.